Effect of sheep diets with macadamia by-product and protected fat on the quality of fresh and frozen semen
Resumo
The objective was to evaluate the seminal quality of Morada Nova sheep consuming diets supplemented with macadamia residue and protected fat. The experiment was carried out with 24 rams aged 18 or 30 months, distributed in four treatment groups: control (C), 50 g (MAC50) or 150 g (MAC150) of macadamia industrial by-product; and 50 g of protected fat (Megalac®), added to the concentrate. Semen was collected at four intervals: before supplementation (day 0), 30, 60 and 75 days after the beginning of supplementation. Semen was collected to measure volume, appearance, motility, vigour, swirling, concentration and morphology. At days 60 and 75, semen was frozen for determination of plasma membrane integrity, acrosome integrity and mitochondrial activity after thawing. Analysis of variance was performed and the means were compared by the SNK test. In the analysis of fresh semen, a significant effect (p < 0.05) of the treatments on motility was observed. For cryopreserved semen, there was no difference (p > 0.05). The inclusion of 50 or 150 g of macadamia residue or 50 g of Megalac in the diet does not alter the quantitative and qualitative aspects of fresh and post-thawed semen.
Referências
ACHEAMPONG-BOATENG, O.; BAKARE, A. G.; MBATHA, K. R. The potential of replacing soy bean oil cake with macadamia oil cake in broiler diets. Tropical Animal Health and Production, v.48, p.1283–1286, 2016. https://doi.org/10.1007/s11250-016-1057-y
ACHEAMPONG-BOATENG, O.; BAKARE, A.G.; NKOSI, D.B.; MABTHA, K.R. Effects of different dietary inclusion levels of macadamia oil cake on growth performance and carcass characteristics in SouthAfrican mutton merino lambs. Tropical Animal Health and Production, v.49, p.733–738, 2017. https://doi.org/ 10.1007/s11250-017-1250-7
ALIZADEH, A.; ESMAEILI, V.; SHAHVERDI, A.; RASHIDI, L. Dietary fish oil can change sperm parameters and fatty acid profiles of ram sperm during oil consumption period and after removal of oil source. Cell Journal, v.16, p.289-298, 2014.
AMARAL, A.; LOURENÇO, B.; MARQUES, M.; RAMALHO-SANTOS, J. Mothocondria functionality and sperm quality. Reproduction, v.146, p.163-174, 2013. https://doi.org/10.1530/rep-13-0178
ARTUNDUAGA, M.A.T.; COELHO, S.G.; BORGES, A.M.; LANA, A.M.Q.; REIS, R.B.; CAMPOS, B.G.; SATURNINO, H.M.; SÁ FORTES, R.V.; COSTA, H.N. Primeira onda folicular e ovulação de vacas primíparas da raça Holandesa alimentadas com diferentes fontes energéticas durante o período de transição. Arquivo Brasileiro Medicina Veterinária e Zootecnia, v.62, p.116-123, 2010. https://doi.org/10.1590/s0102-09352010000100016
BARTH, A.D.; OKO, R. J. Abnormal morphology of bovine spermatozoa. Ames: Iowa State University Press, 1989. 285p.
BIANCHI, A.E.; MACEDO, V.P.; FRANÇA, R.T.; LOPES, S.T.A.; LOPES, L.S.; STEFANI, L.M.; VOLPATO, A.; LIMA, H.L.; PAIANO, D.; MACHADO, G.; DA SILVA, A.S. Effect of adding palm oil to the diet of dairy sheep on milk production and composition, function of liver and kidney, and the concentration of cholesterol, triglycerides and progesterone in blood serum. Small Ruminant Research, v.117, p.78-83, 2014. https://doi.org/10.1016/j.smallrumres.2013.12.025
CÂMARA, D.R.; SILVA, S.V.; ALMEIDA, F.C.; NUNES, J.F.; GUERRA, M.M.P. Effects of antioxidants and duration of pre-freezing equilibration on frozen-thawed ram semen. Theriogenology, v.76, p.341-350, 2011. https://doi.org/10.1016/j.theriogenology.2011.02.013
COSTA, R.L.D.; FONTES, R.S.; CUNHA, E.A.; BUENO, M.S.; QUIRINO, C.R.; AFONSO, V.A.C.; OTERO, W.G.; SANTOS, L.E.; DIAS, A.B. Reproductive performance of Santa Inês ewes fed protected fat diet. Pesquisa Agropecuária Brasileira, v.46, p.663-668, 2011. https://doi.org/10.1590/s0100-204x2011000600013
ESMAEILI, V.; SHAHVERDI, A. S.; ALIZADE, A.R.; ALIPOUR, H.; TOWHIDI, A.; ZARRABI, M. Fatty Acid Profiles of Ram's Sperm after Removing Some Fatty Acid Sources from the Diets and Persistency of Fatty Acids in Sperm. Journal of Fertility and Sterility, v.5, p.211-216, 2012.
FARAJI, Z.; ZAMIRI, M. J.; ROWGHANI, E.; AKHLAGHI, A.; JAFARZADEH, M. R.; BAYAT, A. R.; GHOLAMI, M. Effect of feeding olive-pulp silage on the seminal characteristics and fatty acid profile of the sperm plasma membrane in Iranian fat-tailed sheep. Small Ruminant Research, v.105, p.216-221, 2012. https://doi.org/10.1016/j.smallrumres.2011.12.003
GERON, L.J.V.; MEXIA, A.A.; GARCIA, J.; SILVA, M.M.; ZEOULA, L.M. Suplementação concentrada para corderios terminados a pasto sobre o custo de produção no período de seca. Semina: Ciências Agrárias, v.33, p.797-808, 2012. https://doi.org/10.5433/1679-0359.2012v33n2p797
GHOLAMI, H.; CHAMANI, M.; TOWHIDI, A.; FAZELI, M.H. Improvement of semen quality in Holstein bulls during heat stress by dietary supplementation of Omega-3 fatty acids. International Journal of Fertility Sterility, v.4, p.160-167, 2011.
GHOLAMI, H.; CHAMANI, M.; TOWHIDI, A.; FAZELI, M.H. Effect of feeding a docosahexaenoic acid‐enriched nutriceutical on the quality of fresh and frozen‐thawed semen in Holstein bulls. Theriogenology, v.74, p.1548–1558, 2010. https://doi.org/10.1016/j.theriogenology.2010.06.025
GOERING, H.K.; VAN SOEST, P.J. Forage fiber analysis (Apparatus, reagents, procedures and some applications). Washington, DC: USDA, Agricultural Handbook, 1970. 379p.
HOMEM JUNIOR, A.C.; EZEQUIEL, J.B.; GALATI, R.L. Grãos de girassol ou gordura protegida em dietas com alto concentrado e ganho compensatório de cordeiros em confinamento. Revista Brasileira de Zootecnia, v.39, p. 563-571, 2010. https://doi.org/10.1590/s1516-35982010000300016
HRUDKA, F. Cytochemical and ultracytochemical demonstratation of cytochrome c oxidase in spermatozoa and dynamics of its changes accompanying ageing orinduced by stress. International Journal of Andrology, v.19, p.809-828, 1987. https://doi.org/10.1111/j.1365-2605.1987.tb00385.x
KANEKO J.J.; HARVEY, D.W.; BRUSS, W.L. Clinical bioquemistry of domestic animals. 5thed. New York: Academic Press, 1997. 932p.
KARGAR, R.; FOROUZANFAR, M.; GHALAMKARI, G.; ESFAHANI, M.H.N. Dietary flax seed oil and/or vitamin E improve parameters of cloned goats following freezing-thawing. Cryobiology, v. 74, p. 110-114, 2017. https://doi.org/10.1016/j.cryobiol.2016.11.007
LOSANO, J.D.A.; ANGRIMANI, D.S.R.; RUI, B.R.; BICUDO, L.C.; DALMAZZO, A.; SILVA, B.C.S.; VIANA, C.H.C.; MENDES, C.M. ; ASSUMPÇÃO, M.E.O.A. ; BARNABE, V.H.; NICHI, M. The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls. Zygote, v. 26, p. 1-8, 2018. https://doi.org/10.1017/s0967199418000096
MIES FILHO, A. Reprodução dos animais domésticos e inseminação artificial. 6. ed. Porto Alegre. RS: Editora Sulina S/A, 1987. 645p.
MORAES, E.A.; TORRES, C.A.A.; GUIMARÃES, J.D.; MURGAS, L.D.S. Efeito de fontes de óleo e níveis de suplementação de vitamina E na ração sobre as características físicas e morfológicas do sêmen in natura de suínos. Arquivos Brasiliero de Medicina Veterinaria e Zootecnia, v.62, p.521-527, 2010. https://doi.org/10.1590/s0102-09352010000300004
MOSES, D.F.; HERAS, M.A.; VALCÁRCEL, A.; PÉREZ, L.; BALDASSARRE, H. Use of computerized motility analyzer for the evaluation of frozen-thawed ram spermatozoa. Andrologia, v.27, p.25–29, 1995. https://doi.org/10.1111/j.1439-0272.1995.tb02091.x
MOTLAGH, M.K.; SHARAFI, M.; ZHANDI, M.; MOHAMMADI-SANGCHESHMEH, A.; SHAKERI, M.; SOLEIMANI, M.; ZEINOALDINI, S. Antioxidant effect of rosemary (Rosmarinus officinalis L) extract in soybean lecithin-based semen extender following freeze-thawing process of ram sperm. Cryobiology, v.69, p.217-222, 2014. https://doi.org/10.1016/j.cryobiol.2014.07.007
POPE, C. E.; ZHANG, Y. Z.; DRESSER, B. L. A simple staining method for evaluating acrosomal status of cat spermatozoa. Journal Zoo Wildlife Medicine, v. 22, p. 87-95, 1991. https://doi.org/10.1016/0093-691x(91)90233-4
SAMADIAN, F.; TOWHIDI, A.; REZAYAZDI, K.; BAHREINI, M. Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm. The Animal Consortium, v.4, p.2017-2022, 2010. https://doi.org/10.1017/s1751731110001308
SAMPAIO, B.F.B.; BENDER, E.S.C.; COSTA-E-SILVA, E.V.; ZÚCCARI, C.E.S.N. Efeito da adição de ácido oleico-linoleico e β-sitosterol ao diluente de congelação do sêmen de touros e garanhões. Semina Ciencias Agrarias, v.36, p.1369-1384, 2015. http://dx.doi.org/10.5433/1679-0359.2015v36n3p1369
SARAGUST, J.; GACITUA, H.; ROZENBOIM, I.; ARAV, A. Protective effects of iodixanol during bovine sperm cryopreservation. Theoriogenology, v.71, p.1425-1432, 2009. https://doi.org/10.1016/j.theriogenology.2009.01.019
SELVARAJU, S.; RAJU, P.; RAO, S.B.; RAGHAVENDRA, S.; NANDI, S.; DINESHKUMAR, D.; THAYAKUMAR, A.; PARTHIPAN, S.; RAVINDRA, J.P. Evaluation of maize grain and pulyunsaturated fatty acid (PUFA) as energy sources for breeding rams based on hormonal, esperm functional parameters and fertility. Reproduction, Fertility and Development, v.24, p.669-678, 2012. https://doi.org/10.1071/rd11229
SICHERLE, C.C.; MAIA,M.S.; BICUDO, S.D.; RODELLO, L.; AZEVEDO, H.C. Lipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen supplemented with catalase or Trolox. Small Ruminant Research, v. 95, p.144–149, 2011. https://doi.org/10.1016/j.smallrumres.2010.10.011
SILVA, S.V.; SOARES, A.T.; BATISTA, A.M.; ALMEIDA, F.C.; NUNES, J.F.; PEIXOTO, C.A.; GUERRA, M.M.P. In Vitro and In Vivo Evaluation of Ram Sperm Frozen in Tris Egg-yolk and Supplemented with Superoxide Dismutase and Reduced Glutathione. Reproduction in Domestic Animals, v.46, p.874-881, 2011. https://doi.org/10.1111/j.1439-0531.2011.01758.x
STAPLES, C.R.; BURKE, J.M.; THATCHER, W.W. Influence of supplemental fats on reproductive tissues and performance of lactating cows. Journal of Dairy Science, v.81, p.856-871, 1998. https://doi.org/10.3168/jds.s0022-0302(98)75644-9
VAN SOEST, P.J.; ROBERTSON, J.B.; LEWIS, B.A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal Dairy Science, v.21, p. 3583-3597, 1991. https://doi.org/10.3168/jds.s0022-0302(91)78551-2
VAN TRAN, L.; MALLA, B.A.; KUMAR, S.; TYAGI, A.K. Polyunsaturated fatty acids in male ruminant reproduction – a review. Asian-Australasian Journal of Animal Sciences, v.30, p.622-637, 2017. https://doi.org/10.5713/ajas.15.1034
WONNACOTT, K.R.; KWONG, W.Y.; HUGHES, J.; SALTER, A.M.; LEA, R.G.; GARNSWORTHY, P.C.; SINCLAIR, K.D. Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos. Reproduction, v.139, p.57-69, 2010. https://doi.org/10.1530/rep-09-0219
Copyright (c) 2019 Boletim de Indústria Animal

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Os autores não serão remunerados pela publicação de trabalhos, pois devem abrir mão de seus direitos autorais em favor deste periódico. Por outro lado, os autores ficam autorizados a publicar seus artigos, simultaneamente, em repositórios da instituição de sua origem, desde que citada a fonte da publicação original seja Boletim de Indústria Animal. A revista se reserva o direito de efetuar, nos originais, alterações de ordem normativa, ortográfica e gramatical, com vistas a manter o padrão culto da língua e a credibilidade do veículo. Respeitará, no entanto, o estilo de escrever dos autores. Alterações, correções ou sugestões de ordem conceitual serão encaminhadas aos autores, quando necessário. Nesses casos, os artigos, depois de adequados, deverão ser submetidos a nova apreciação. As opiniões emitidas pelos autores dos artigos são de sua exclusiva responsabilidade. Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob a Licença Creative Commons Attribution (CC-BY-NC). A condição BY implica que os licenciados podem copiar, distribuir, exibir e executar a obra e fazer trabalhos derivados com base em que só se dão o autor ou licenciante os créditos na forma especificada por estes. A cláusula NC significa que os licenciados podem copiar, distribuir, exibir e executar a obra e fazer trabalhos derivados com base apenas para fins não comerciais.