E. F. Ferraz, D. L. Plut, O. S. Leite.
Enzimas glicolíticas em espermatozóides de bovinos da raça Holandesa: recomendação de métodos.
Foram determinadas as atividades da hexoquinase,
fosfoglicoisomerase, piruvatoquinase e lactato desidrogenase em espermatozóides
de bovinos da raça holandesa, empregando-se uma metodologia simplificada,
comumente usada em
eritroenzimologia. Em vista da obtenção de baixos
coeficientes de variação, o que indica boa reprodutibilidade dos resultados, essa
metodologia é recomendada para o estudo das atividades enzimáticas em
espermatozóides daquela espécie.
Glycolytic enzymes in spermatozoa from Holstein bulls:recommendation of methods.
of this investigation was to evaluate the suitability of simplified methods,
commonly used in evrythroenzymology, for the study of enzymatic activities in
spermatozoa; this would permit to establish standards of activities in such
cells, for the study of their metabolic behaviour in different physiological
and experimental conditions. Thus, the activities of hexokinase,
phosphoglucoisomerase pyruvate kinase and lactate dehydrogenase were determined
in sperrnatozoa from Holstein bulls, using
nine ejaculates collected from three bulis. The results showed that the methods
employed haci good reproductibility, since the variation coefficients of the
activities for enzyme were low. It was concluded that the methodology employed
may be recommended for establishing standards of enzymatic activities in bull
spermatozoa. The values obtained in this paper, however, cannot be considered
as representative of a population, since the number of animals was not
sufficient for that purpose.