Autores
Andiara Moraes Evangelista Barbieri; Helder Louvandini, Rafael Canonenco de Araujo, Ricardo Lopes Dias da Costa, Bruno Ceneviva Fornazari, Luciana Morita Katiki
Formulations containing essential oils: potential for anthelmintic activity against sheep gastrointestinal nematode
Resumo
Improper use of chemicals for the Haemonchus contortus control resulted in the emergence of resistant
strains. The combination of two or more bioactives, synthetic or natural, have
superior effects when in comparison to single compounds, resulting in reduced doses
and low toxicity of the user. In this study, Cinammaldehyde, Carvone, Carvacrol
and Anethole essential oils were combined as follow: 1) Cinnamaldehyde (25%) +
Carvone (50%) + Anethole (25%), 2) Cinnamaldehyde (50%) + Carvone (25%) +
Carvacrol (25%) and 3) Cinnamaldehyde (25%) + Carvacrol (25%) + Anethole (25%)
+ Carvone (25%). Each formulation (12.48 ml) was solubilized
with detergent Tween 80 (100 µl) and added 5887.52 ml of distilled water
to make the main solution for in vitro hatchability test of H. contortus eggs. Decreasing
concentrations of these formulations were tested (2.08 mg/ml; 1.04 mg/ml; 0.39
mg/ml; 0.15 mg/ml; 0.06 mg/ml; 0.02 mg/ml; and 0.007 mg/ml) in 100 eggs of H. contortus (6 replicates) in 48-well
plate, and incubated for 24 hours at 27° C to evaluate their effectiveness.
Data were analyzed with SAS Probit (SAS Inst., Inc., Cary, NC) estimating
the LC50 and the independent variables (Table 1). It was concluded
that the formulation 1 had the lowest LC50 of 0.020 mg/ml,
suggesting better anthelmintic efficacy, followed by formulation 2, LC50
of 0.028 mg/ml and formulation 3 that showed worst efficacy with LC50
of 0.045 mg/ml.
Potencial anti-helmíntico de formulações contendo óleos essenciais em nematóides gastrintestinais de ovinos
Abstract
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