Autores
Andiara Moraes Evangelista Barbieri; Bruno Ceneviva Fornazari, Erika Breda Canova, Everton Luis Moreira, Luciana Morita Katiki
Effectiveness of Areca catechu Linn against Haemonchus contortus in vitro egg hatch assay
Resumo
Areca
catechu Linn belongs to Palm Arecaceaes family, popularly known as Betel palm, originated from
tropical countries of Oceania and East Africa.
Popularly known for its wide pharmacological activity, Areca catechu has also antibacterial, antihelmintic, antioxidant
and detoxifying activity. In its composition is found carbohydrates, fibers,
fats, flavonoids, tannins, alkaloids and minerals, and its nut is the main
product with medicinal use. In this study, we tested the action of A. catechu L. on in vitro hatchability of eggs of sheep gastrointestinal nematode Haemonchus contortus. The seed was
broken and dried at 37°C,
crushed in 2 mm
size and its contents were extracted with a solution of acetone:water (70:30),
filtered, rotoevaporated and freeze dried. For the main solution it was used
300 mg of plant extract, mixed at 2850 µl of distilled water and DMSO (150 µl),
that was diluted in decreasing concentrations (50 mg/ml, 25 mg/mL, 12.5 mg/ml,
6.25 mg/ml, 3.12 mg/ml, 1.56 mg/ml and 0.78 mg/ml). A hundred eggs of H. contortus were added to different
concentrations of A. catechu extract in
48-well plate, and incubated for 24 hours at 27°C to evaluate their
effectiveness. Data were analyzed with SAS Probit (SAS Inst., Inc., Cary, NC)
estimating the LC50 and the independent variables (dose). The
results showed that at doses of 7.45 mg/mL can inhibit 50% of the hatchability
of eggs of H. contortus (Figure 1).
Eficácia de Areca catechu Linn contra Haemonchus contortus em teste de eclodibilidade in vitro
Abstract
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